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Plasmid

Part:BBa_K1456017:Design

Designed by: Commercial   Group: iGEM14_ATOMS-Turkiye   (2014-10-17)


pTET-off Vector


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 766
    Illegal XbaI site found at 777
    Illegal SpeI site found at 103
    Illegal PstI site found at 5366
    Illegal PstI site found at 6289
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 766
    Illegal NheI site found at 6824
    Illegal SpeI site found at 103
    Illegal PstI site found at 5366
    Illegal PstI site found at 6289
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 766
    Illegal BglII site found at 6502
    Illegal BamHI site found at 1791
    Illegal BamHI site found at 4482
    Illegal XhoI site found at 1
    Illegal XhoI site found at 4470
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 766
    Illegal XbaI site found at 777
    Illegal SpeI site found at 103
    Illegal PstI site found at 5366
    Illegal PstI site found at 6289
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 766
    Illegal XbaI site found at 777
    Illegal SpeI site found at 103
    Illegal PstI site found at 5366
    Illegal PstI site found at 6289
    Illegal NgoMIV site found at 2216
    Illegal NgoMIV site found at 5555
    Illegal NgoMIV site found at 5838
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3545
    Illegal SapI site found at 2462
    Illegal SapI site found at 5778
    Illegal SapI site found at 5988


Design Notes

none


Source

none

References